Supplementary Figure 5 from Chimeric Antigen Receptor T Cells with Dissociated Signaling Domains Exhibit Focused Antitumor Activity with Reduced Potential for Toxicity In Vivo
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 854K, Figure S5. F-28z CART cells produce dramatically less IFN-γ in response to stimulation with A1847 (M+/F-) cells compared to A1847 (M+/F+) cells. A. F-28z CAR expression on human CD3+ T cells upon transduction with lentivirus compared to untransduced T cells. F-28z CART transduced T cells were detected using biotinylated-protein L followed by SA-PE. B. Transduced T cells (1 x 105 T cells) were cultured alone (none) or stimulated overnight with an equal number of either FRa-negative PEO-1 cells or A1847 (M+/F-) cells, or FRa-positive A1847 (M+/F+) cancer cells. Cell-free supernatant was harvested after ~20 hours of incubation and the IFN-γ levels were measured with ELISA. Mean IFN-γ concentration plus-minus SEM (pg/ml) from triplicate cultures is shown. ***P < 0.001 comparing the IFN-γ secretion produced by M-z/F-28 CART cells against A1847M+/F+ versus A1847M+/F- or PEO cells.
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362551255