Supplementary Fig. S9 from Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Year: 2023
Type: supplementary-materials
Abstract: Evaluation of inflammatory cytokines and ICAM-1 in tumors of mice treated with PEGPH20/shIDO-ST. Mice bearing 14 day orthotopic KPC tumors were treated with indicated regimens. Tumors were excised 96 hours post-treatment and used for RT-qPCR to detect cytokines or immunofluorescence to detect ICAM-1. Data are representative of multiple experiments. (A) Total mRNA was isolated (Qiagen) to generate cDNA libraries using a RevertAid First Strand DNA kit (Thermo Scientific) and analyzed for indicated cytokine mRNAs (Power Syber Green kit, 7300 RT-PCR cycler (Applied Biosystems)). Experimental Ct values were normalized to GAPDH. Relative expression levels were calculated using the equation 2-delta deltaCt and using the CT values of the PEGPH20 only treatment group as the comparator . *p{less than or equal to}0.05, **p{less than or equal to}0.01, ***p{less than or equal to}0.001 using 1-way ANOVA. (B) Tissue sections from indicated treatment groups were stained for ICAM-1 (green), PMN (red) and nuclei (DAPI, blue). Arrows represent leading edges of PMN infiltration associated with ICAM-1. Magnification, 10X.
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362551951