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Supplementary Fig. S12 from Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors

Year: 2023

Type: supplementary-materials

Abstract: Western blot analysis of cleaved caspase 3 (CC3). Mice bearing 14 day orthotopic KPC tumors were treated with indicated regimens. Tumors were excised 96 hours post-treatment, homogenized, and total protein was extracted using cell lysis buffer containing a protease inhibitor cocktail mix (Roche). Equal amounts of protein (Bradford) were loaded. Blots were stained separately for pro-caspase 3 and CC3. Equal loading of samples was confirmed by stripping CC3 membranes and reprobing for β-actin. Data are representative of multiple experiments.

DOI: 10.1158/2326-6066.22537520

Authors: Edwin R. Manuel, Jeremy Chen, Massimo D'Apuzzo, Melanie G. Lampa, Teodora I. Kaltcheva, Curtis B. Thompson, Thomas Ludwig, Vincent Chung, Don J. Diamond

Institutions: City Of Hope National Medical Center, City of Hope, Beckman Research Institute, Halozyme Therapeutics (United States), The Ohio State University

Language: English

Topic: Cancer Research and Treatments

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362551972

Cites: 43

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Related to: 10