Supplementary Figure 1 from PD-1 Expression on Peripheral Blood Cells Increases with Stage in Renal Cell Carcinoma Patients and Is Rapidly Reduced after Surgical Tumor Resection
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 4006K, Myeloid cell gating strategy. The myeloid cell gating strategy used in these experiments is shown with representative data from a healthy control donor. A. Forward scatter area and height were used to exclude aggregates and clusters of cells. B. Dead cells were excluded by propidium iodode staining. C. CD45+ Leukocytes were divided into lymphocyte and myeloid gates based on side scatter. D. T cells and NK cells were excluded from the myeloid cell analysis by CD3 and CD56 expression. E. Cells that express neither CD14 nor CD16 were excluded from the myeloid analysis (mostly B cells at this point in the gating). F. Myeloid cells were sub-gated into Classical Monocytes (CD14bright CD16-), Intermediate Monocytes (CD14bright CD16dim), Non-classical Monocytes (CD14dim CD16dim), CD14bright CD16bright myeloid cells, and Neutrophils (CD14dim CD16bright). Note that most of the neutrophils were removed along with the erythrocytes during gradient centrifugation on Lympoprep).
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362552895