Supplementary Figures and Legends from Secretory IgM Exacerbates Tumor Progression by Inducing Accumulations of MDSCs in Mice
Year: 2023
Type: supplementary-materials
Abstract: S1. Crosslinking and preparation of oligomeric HEL. S2. Oligomeric HEL crosslinks the BCR, and induces robust BCR signal transduction in MD4+/-/Eμ-TCL1+/+ B cells. S3. Oligomeric HEL activates a more rapid BCR signaling than F(ab')2 in MD4+/- and MD4+/-/Eμ-TCL1+/+ B cells. S4. CD11b+/Ly6G+ granulocytic cells or CD11b+/Ly6C+ monocytic cells do not accumulate in the blood and spleens of 6-month-old WT, MD4+/- and μS-/- mice. S5. B cells and CLL cells from MD4+/-/Eμ-TCL1+/+ and Eμ-TCL1+/+ but not μS-/-/Eï�-TCL1+/+ mice produce sIgM; human CLL cells also produce and secrete IgM. S6. Decreased percentages of CD11b+/Ly6G+ granulocytic cells in spleens of 6-week-old μS-/-/Eï�-TCL1+/+ mice; decreased percentages of CD11b+/Ly6G+ granulocytic cells and CD11b+/Ly6C+ monocytic cells in the bone marrow of 6-month-old μS-/-/Eμ-TCL1+/+ mice; increased percentages of CD11b+/Ly6G+ granulocytic cells and CD11b+/Ly6C+ monocytic cells in the bone marrow of 6-week-old MD4+/-/Eμ-TCL1+/+ mice; and increased percentages of CD11b+/Ly6G+ granulocytic cells in the bone marrow of 6-month-old MD4+/-/Eμ-TCL1+/+ mice. S7. CD11b+/Ly6G+ granulocytic cells can be purified from the spleens and peripheral blood of MD4+/-/Eμï�-TCL1+/+ mice. S8. CD11b+/Ly6G+ granulocytic cells purified from the spleens or bone marrow of LLC-grafted WT and μS-/- mice have small or little effect in suppressing T cell proliferation.
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362566699