Supplementary Figures S1-S11 from Entinostat Converts Immune-Resistant Breast and Pancreatic Cancers into Checkpoint-Responsive Tumors by Reprogramming Tumor-Infiltrating MDSCs
Year: 2023
Type: supplementary-materials
Abstract: This file contains supplemental figures that includes: FACS gating strategies; QC plots for NanoString cell typing; survival and tumor growth of neu-N mice receiving ENT and ICIs without ï�¡-HER2; circulating MDSCs and absolute numbers of G-MDSCs infiltrating neu-N and Panc02 tumors; G-MDSCs quantified using flow cytometry from neu-N tumors or from hepatic Panc02 metastases; MDSC functional markers by flow cytometry and expression of PD-L1 on G-MDSCs; patterns of myeloid cell functional markers on gene expression profiling; qPCR validation of NanoString, and T cell proliferation and IFNï�§ï€ production within the TME; western blots depicting protein expression levels of STAT3 and phospho-STAT3 and representative proteins from NanoString pathways; the exhaustion state of CD8+ T cells.
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362566825