Supplementary Methods, Figure Legends from The Promotion of Breast Cancer Metastasis Caused by Inhibition of CSF-1R/CSF-1 Signaling Is Blocked by Targeting the G-CSF Receptor
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Materials and Methods Flow cytometryLungs and tumors were digested with collagenase I (CLS-1; Worthington, USA) in Dulbecco's Modified Eagle Medium (DMEM; Invitrogen-GIBCO).Cells (2x10 5 or 20µl of blood) were washed twice in PBS, counted, incubated with 25% mouse serum at 4ºC for 20 min, washed twice in PBS and incubated with primary antibodies or appropriate isotype
Authors: Agnieszka Swierczak, Andrew D. Cook, Jason C. Lenzo, Christina M. Restall, Judy P. Doherty, Robin L. Anderson, John A. Hamilton
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362567084