Figure S2 from BRCA1/MAD2L1 Deficiency Disrupts the Spindle Assembly Checkpoint to Confer Vinorelbine Resistance in Mesothelioma
Year: 2023
Type: other
Abstract: Silencing of BRCA1 and MAD2L1 leads to resistance to docetaxel. A) 24 hours following transfection, cells were treated with docetaxel 20 nM for a further 24 hours and caspase 3 activity measured. Data were normalised to untreated siNT control. MSTO-211H: siNT NT vs siNT DOCE p=0.0003; siBRCA1 NT vs siBRCA1 DOCE p=0.0206; siNT DOCE vs siBRCA1 DOCE p=0.0013. REN: siNT NT vs siNT DOCE p<0.0001; siBRCA1 NT vs siBRCA1 DOCE p=0.0069; siNT DOCE vs siBRCA1 DOCE p<0.0001. B) Cells were treated with docetaxel 20 nM for 24 hours and caspase 3 activity measured. Data were normalised to parental cells. MSTO-211H NT vs MSTO-211H DOCE p<0.0001; MSTO211H DOCE vs MVR DOCE p<0.0001. REN NT vs REN DOCE p<0.0001; REN VNB vs RVR VNB p<0.0001; RVR NT vs RVR DOCE p=0.0069. C) The cells indicated were treated with 20 nM docetaxel before time-lapse imaging using phase contrast microscopy. Cells were imaged every 15 mins for 25 h following addition of drug. Control cells were treated with DMSO. Stills from representative movies are shown. Scale bar 200μm.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362595957