Supplementary Figure 2 from Targeting Plasminogen Activator Inhibitor-1 Inhibits Angiogenesis and Tumor Growth in a Human Cancer Xenograft Model
Year: 2023
Type: supplementary-materials
Abstract: PDF - 1338KB, A, About 103 urothelial cells (T24-PAI-1KD-19, T24-PAI-1KD-22, UM-UC-14-PAI-1KD-4, UM-UC-14-PAI-1KD-17) were seeded in 6 well plates and incubated for 14 days. After 14 days, colonies were fixed with 6.0% glutaraldehyde, stained with 0.5% crystal violet and counted under light microscopy. Data were represented as mean + SD of three independent replicates. B, About 2 x 103 urothelial cells (T24-PAI-1KD-19, T24-PAI-1KD-22, UM-UC-14-PAI-1KD-4, UM-UC-14-PAI-1KD-17) were mixed in agar solution and fresh complete media and seeded in 96 well plates. Cells were incubated for 6-8 days. Colony formation was quantified using the fluorescent cell stain CyQuant GR Dye (Cell Biolabs Inc., San Diego, CA) in the FLUOstar Optima Reader. Data were represented as mean + SD of three independent replicates. All experiments were repeated at least three times. Targeting PAI-1 was noted to retard colony formation. *, p < 0.05.
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362609198