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Figure S2 from A Small-Molecule Inhibitor of WEE1, AZD1775, Synergizes with Olaparib by Impairing Homologous Recombination and Enhancing DNA Damage and Apoptosis in Acute Leukemia

Year: 2023

Type: other

Abstract: AML and ALL cell lines display elevated homologous recombination in response to olaparib treatment. A-E. MV;411 (A), Molm13 (B), Jurkat (C), REH (D), and OCI-AML3 (E) cells received no treatment (NT) or were treated with olaparib (2 uM) for 72 hr then fixed and stained with anti-Rad51 (primary antibody), Alexo Fluor 488 conjugated secondary antibody, and DAPI. Representative images are shown. Rad51 foci per cell were quantified and are displayed to the right of the images. Results are shown and mean {plus minus} SEM calculated from a minimum of 50 cells per condition. ***, P < 0.001. ****, P < 0.0001.

DOI: 10.1158/1535-7163.22508044

Authors: Tamara B. Garcia, Jonathan C. Snedeker, Dmitry Baturin, Lori Gardner, Susan P. Fosmire, Chengjing Zhou, Craig T. Jordan, Sujatha Venkataraman, Rajeev Vibhakar, Christopher C. Porter

Language: English

Topic: PARP inhibition in cancer therapy

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362610220

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