Supplementary Figure 3 from A Modified HSP70 Inhibitor Shows Broad Activity as an Anticancer Agent
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 395K, A) Binding assays of H1299 cells transfected with HA-tagged full-length and truncated HSP70 encoding the amino acids (aa) shown, treated with 20 uM biotinylated PES (B-PES) and 20 uM PES-Cl (PES-Cl). Biotinylated PES complexes were precipitated with avidin beads, eluted and detected with an anti-HA antibody following imunoblotting. Input is shown on the top panel, immunoprecipitation (IP) with avidin is depicted on the bottom panel. B) Cell viability of WI38 non-transformed fibroblasts after 48 hour treatment with 10 uM PES and PES-Cl. The values shown are the averaged results from three independent experiments, and error bars mark standard deviations. C) Western blot analysis of HSP90 client proteins in soluble cell extract following 48 hours of treatment with 10 uM PES-Cl. Actin is shown as a loading control. D) In silico docking of PES-Cl to the substrate binding domain of HSP70.
Language: English
Topic: Heat shock proteins research
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362610443