Work

Supplementary Figures 1-6 from O-GlcNAcylation Enhances Double-Strand Break Repair, Promotes Cancer Cell Proliferation, and Prevents Therapy-Induced Senescence in Irradiated Tumors

Year: 2023

Type: supplementary-materials

Abstract: S1. Validation of TagRFP-IBD as IRIF reporter in vitro. S2. Effects of radiation on MCF7 cells with altered O-GlcNAcylation. S3. Targeting O-GlcNAc modification by small molecule inhibitors affects kinetics of IRIF resolution in different cell lines. S4. Cellular apoptosis assays using fluorescent dyes and a caspase substrate probe. S5. Alloxan potentiates senescence in Panc02 cells in a radiation dose-dependent manner. S6. Blocking O-GlcNAcylation in vivo by inducing shOGT or alloxan treatment qualitatively reduces EZH2 immunoreactivity after irradiation.

DOI: 10.1158/1541-7786.22512484

Authors: Elena V. Efimova, Oliver K. Appelbe, Natalia Ricco, Steve S.-Y. Lee, Yue Liu, Donald J. Wolfgeher, Tamica N. Collins, Amy C. Flor, Aishwarya Ramamurthy, Sara Warrington, Vytautas P. Bindokas, Stephen J. Kron

Language: English

Topic: Glycosylation and Glycoproteins Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362610642

Cites:

Cited by:

Related to: 10