Figure S2 from Induced Telomere Damage to Treat Telomerase Expressing Therapy-Resistant Pediatric Brain Tumors
Year: 2023
Type: other
Abstract: A, Telomerase activity in HFF+hTERT cells treated with DMSO, 6-thio-dG (3 µM for 3 days), and imetelstat (IMT; 2 µM). For combination treatment, cells were pre-treated with IMT for 3 days and then with 6-thio-dG for another 3 days. (-) C, (+) C, and IC indicate negative control, positive control, and internal control respectively. Telomerase products are indicated. B-C, effect of telomerase inhibition on cell growth of HFF (B) and HFF+hTERT (C) cells treated with imetelstat (IMT; 2 µM) only, 6-thio-dG (3 µM) only, or 6-thio-dG in combination with imetelstat as described in A. Error bars represent the standard deviation generated from triplicates. P-value, **, P<0.01. D, quantification showing percent cells with TIFs in HFF+hTERT cells treated with imetelstat (IMT; 2 µM) only, 6-thio-dG (3 µM for 3 days), or in combination with imetelstat as described in A. Cells counted for each treatment (n= ~50 cells). Bottom panels show representative images showing TRF2 (green), γH2AX (red), and TIFs (yellow) indicated by white arrows. DAPI (blue) indicates nucleus staining.
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362611730