Supplementary Figures 1 - 9 from Hypoxic Stress Facilitates Acute Activation and Chronic Downregulation of Fanconi Anemia Proteins
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 1436KB, S1. FANCD2 is ubiquitinated and transcriptionally down-regulated upon treatment with DFX in MCF7 cells. S2. FANCD2 is ubiquitinated and transcriptionally down-regulated upon treatment with DFX in A549 cells. S3. FANCD2 and FANCI ubiquitination does not occur at less severe levels of hypoxia. S4. siRNA depletion of ATR, but not ATM, inhibits FANCD2 and FANCI ubiquitination induced by hypoxia and DFX. S5. FANCD2 and FANCI ubiquitination upon DFX treatment is independent of HIF-1. S6. FANCD2 protein down-regulation by DFX is not due to altered protein stability and transcriptional down-regulation may be related to p130 dephosphorylation and HIF-2. S7. FANCD2 protein level decreases in hypoxia and is restored by HPV16-E7 overexpression. S8. FANCI transcriptional down-regulation upon prolonged exposure to DFX or hypoxia is mitigated by HPV16-E7 overexpression. S9. Increased chromosomal aberrations induced by hypoxia in the absence of FANCD2 are not detected by cytogenetic analysis.
Authors: Susan E. Scanlon, Peter M. Glazer
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362612914