Supplementary Figures 1 - 5, Tables 1 - 4 from WHSC1 Promotes Oncogenesis through Regulation of NIMA-Related Kinase-7 in Squamous Cell Carcinoma of the Head and Neck
Year: 2023
Type: supplementary-materials
Abstract: Figure S1. Chromatin immunoprecipitation assays (ChIP) performed in UD-SCC-2 cells treated with siWHSC1 for 3 days. Real-time PCR analysis was conducted using primer pairs as described under "Materials and Methods". Immunoprecipitation was performed with ChIP grade anti-H3K36me2 (Millipore, 07-369) antibodies. Y-axis shows a percentage of the input chromatin. Results are the mean {plus minus}SD of three independent experiments, and P-values were calculated using Student's t-test (n.s.: not significant). Figure S2. WHSC1 protein expression of three SCCHN (FaDu, HN-5 and 93VU147T) cells. Nuclear extracts were immunoblotted with anti-WHSC1 (Abcam, ab75359) and anti-histone H3 (Abcam, ab1791) antibodies. Histone H3 was served as an internal control. Figure S3. Heatmap of down regulated-genes at 48 h and 72 h of WHSC1 knockdown in UD-SCC-2 cells. Figure S4. Validation of WHSC1, HIK3 and MAPK8 expressions in HPV-positive (UD-SCC-2) cells by quantitative real-time PCR 48 or 72 h after treatment with siRNAs. Figure S5. Cross-cancer alteration summary for NEK7. The data were obtained from the TCGA database (http://www.cbioportal.org/public-portal/). Table S1. Characteristics of SCCHN cell lines. Table S2. Primer Sequences for Quantitative RT-PCR. Table S3. siRNA Sequences. Table S4. Gene Ontology analysis of candidate genes.
Language: English
Topic: Head and Neck Cancer Studies
Subfield: Otorhinolaryngology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362536786