Supplementary Figure 3 from RhoC Is an Unexpected Target of RhoGDI2 in Prevention of Lung Colonization of Bladder Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure 3. Depletion of RhoC with a second independent shRNA A. Doubling time for T24T cells expressing shCont or shRhoC-2 was determined by counting cells at 24 h intervals for 96 h. Bargraph shows the mean doubling time{plus minus}s.e. (n=3). *P<0.05. B. Colony formation in soft agar was assessed in T24T cells expressing shCont or shRhoC-2. Bargraph shows the number of colonies formed and is presented as the mean{plus minus}s.e. *P<0.05. 3 independent experiments were performed in triplicate. C. Graph representing incidence of lung colonization upon tail vein injection of T24T cells expressing shControl or shRhoC-2. p=0.66, Fisher's exact test. D. Scatter plot comparing lung burden in animals injected with T24T cells expressing shControl or shRhoC-2 as measured by human DNA-specific qPCR amplification of lung genomic DNA. Mean is indicated. p=0.016, analysis of non-zero values only, Mann Whitney test.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362614207