Supplementary Figures S1 - S9 from PARP1-Driven Poly-ADP-Ribosylation Regulates BRCA1 Function in Homologous Recombination–Mediated DNA Repair
Year: 2023
Type: supplementary-materials
Abstract: This file includes Supplementary Figures S1-S9. Figure S1. PARP1 is a partner of the RAP80-BRCA1 complex and promotes BRCA1 PARsylation. Figure S2. In vitro PARP1-driven PARsylation of BRCA1 fragments. Figure S3. Identification of a BRCA1 sequence required for optimal mono- and poly- ADP-ribosylation of the BRCA1 F3.7 fragment. Figure S4. The BRCA1 D5 sequence is not required for IRIF localization of BRCA1 in S/G2 phase nuclei. Figure S5. Olaparib treatment leads to increased BRCA1 chromatin association at sites near an I-SceI induced DSB. Figure S6. (a) RAP80 depletion and/or Olaparib treatment does not interfere with the interaction between BARD1 and BACH1, CtIP or RAD51. (b) The BRCA1-D5 sequence is required for a stable interaction between BRCA1 and RAP80 after IR, but not for the interaction between BRCA1 and BACH1 or CtIP. (c) RAP80 is not required for BRCA1 PARsylation in cells. (d) The RAP80-BRCA1 complex contains both PARsylated and unmodified BRCA1 molecules. Figure S7. PID is required for efficient RAP80 binding to PAR and PARsylated BRCA1, but not required for RAP80 IRIF localization. Figure S8. PARP1 activity is required for normal HRR tuning. Figure S9. Effects of RAD51 or EXO1/DNA2L depletion on STGC of cells expressing endogenous BRCA1, BRCA1-WT or BRCA1-D5, or depleted of BRCA1.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362616416