Work

Figure S1-S7 and Table S1 from ABC294640, A Novel Sphingosine Kinase 2 Inhibitor, Induces Oncogenic Virus–Infected Cell Autophagic Death and Represses Tumor Growth

Year: 2023

Type: other

Abstract: Figure S1. KSHV infection up-regulates SphK2 expression from endothelial cells. Figure S2. ABC294640 significantly increases endogenous total ceramides/dh-ceramides from TIVE-LTC but not TIVE cells. Figure S3. Chloroquine effectively blocks autophagy induced by ABC294640 in TIVE-LTC cells. Figure S4. The top 2 scored (by the number of pathways) AN networks from 'common' gene sets altered within ABC294640-treated TIVE-LTC. Figure S5. ABC294640 causes G0/G1 cell cycle arrest in TIVE-LTC. Figure S6. Directly silencing of SphK2 by RNAi increases EGR1 expression in TIVE-LTC cells. Figure S7. Successful "knock-down" LC3B and Atg5 from TIVE-LTC cells. Table S1. Primer sequences for qRT-PCR.

DOI: 10.1158/1535-7163.22503679

Authors: Lu Dai, Aiping Bai, Charles D. Smith, Paulo C. Rodriguez, Fangyou Yu, Zhiqiang Qin

Language: English

Topic: Sphingolipid Metabolism and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362482096

Cites:

Cited by:

Related to: 10