Supplemental Figures 1-4 from AKR1C3 Promotes AR-V7 Protein Stabilization and Confers Resistance to AR-Targeted Therapies in Advanced Prostate Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Figure 1: C4-2B parental and C4-2B MDVR tumors were processed and whole tumor lysates were extracted and subjected to western blot; Supplemental Figure 2: The KLK3 mRNA expression and PSA expression in C4-2B parental and C4-2B MDVR cells were determined by qRT-PCR and PSA ELISA respectively; Supplemental Figure 3: Eight C4-2B AKR1C3 stable clones were picked and whole cell lysates were extracted and subjected to western blot; Supplemental Figure 4: AR-V7 was knocked down in LNCaP-neo cells, cells were then treated with 20 µM enzalutamide for 5 days and total cell number was determined.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362622964