Supplementary Methods and Supplementar Figures S1-S13 from Small Molecules Target the Interaction between Tissue Transglutaminase and Fibronectin
Year: 2023
Type: supplementary-materials
Abstract: Materials, methods; Figure S1. 1H NMR spectrum of MT-1; Figure S2. 1H NMR spectrum of MT-2; Figure S3. 1H NMR spectrum of MT-3; Figure S4. 1H NMR spectrum of MT-4; Figure S5. 1H NMR spectrum of MT-5; Figure S6. 1H NMR spectrum of MT-6; Figure S7. Enthalpy kinetics of MT-4 -TG2 interaction as measured by Isothermal Titration Calorimetry (ITC); Figure S8. Effects of TG-53 and MT-4 on TGase activity; Figure S9. TIRF analysis of β1 integrin receptors distribution upon cell treatment with SMIs at the indicated concentrations and adhesion onto FN coated plates; Figure S10. Flow cytometry analysis of integrin β1 plasma membrane expression; Figure S11. Dose response effects induced by TG2-FN inhibitors on FAK phosphorylation; Figure S12. Experimental design for in vivo assay testing the activity of MT4; Figure S13. Cell cycle distribution and apoptosis of OVCAR5 cells primed with MT-4 or TG53 for 72 hours before treatment with paclitaxel (PTX).
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362609835