Supplementary Figure 1 from A Modified HSP70 Inhibitor Shows Broad Activity as an Anticancer Agent
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 130K, A) Chemical structure of biotinylated PES B) Chemical structure of PES and derivatives NSC85560, NSC166772 and NSC303578 (nomenclature is from the National Cancer Institute Drug Repository). C) Cell viability of H1299 and PANC1 cells after 48 hour treatment with 10 uM PES, NSC85560 (855600), NSC166777 (16677) and NSC303578 (303578). Data are the averaged results from three independent experiments, and error bars mark standard deviations. D) Immunoblot analysis of H1299 cells following treatment with 10 uM of the compounds indicated for the timepoints indicated, using antisera to the autophagy (p62SQSTM1 and LC3) and apoptosis (cleaved lamin A) markers indicated, as well as HSP70 and actin (control). The arrows mark monomeric and oligomeric forms of p62SQSTM1, as well as cleaved and lipidated forms of LC3 I and II.
Language: English
Topic: Heat shock proteins research
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362623984