Supplementary Figures from Inhibition of TWIST1 Leads to Activation of Oncogene-Induced Senescence in Oncogene-Driven Non–Small Cell Lung Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures PDF file - 3634K, S1 TWIST1 specific shRNAs suppress TWIST1 transcript and protein levels. S2 Mouse Twist1 can rescue the anti-proliferative effects of knockdown of human TWIST1 in A549 cells. S3 Growth inhibition by silencing TWIST1 is not p53-dependent in KRAS mutant NSCLC. S4 Growth inhibition by silencing TWIST1 is not p21-dependent in KRAS mutant NSCLC. S5 Growth inhibition by silencing TWIST1 is not dependent on induction of p27 in KRAS mutant NSCLC S6 Overexpression of SKP2 does not rescue loss of TWIST1 in KRAS mutant NSCLC. S7 Microarray analysis of three KRAS mutant NSCLC cell lines (H460, H727 and H358) six days after silencing of TWIST1 reveals a striking cell cycle arrest gene signature. S8 Enrichment plots after Gene Set Enrichment Analysis for A. ROSTY_CERVICAL_CANCER_PROLIFERATION_CLUSTER (left) CROONQUIST_IL6_DEPRIVATION_DN (middle) CHANG_CYCLING_GENES (right) B. KANG_DOXORUBICIN_RESISTANCE_UP C. BLUM_RESPONSE_TO_SALIRASIB DN (left) CROONQUIST_NRAS_SIGNALING_DN (right) D. FUJII_YBX1_TARGETS_DN following GSEA performed on shScram and shTWIST1 samples from three KRAS mutant cell lines (H460, H727 and H358) six days after TWIST1 knockdown samples (NOM p-values, FDR qvalues, and FWER p-values were all <0.001 for all gene sets)
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362624504