Supplementary Figure S1 from Relief of Feedback Inhibition of HER3 Transcription by RAF and MEK Inhibitors Attenuates Their Antitumor Effects in BRAF-Mutant Thyroid Carcinomas
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S1 PDF file - 51K, A, Lineage-specific differences in effects of PLX4032 on RAS/RAF/MAPK signaling. DUSP5 expression kinetics in a panel of BRAF wild type (Top) and BRAF mutant (Bottom) cell lines treated with 2 �gM PLX4032 for 1, 6 or 48 h. Points represent DUSP5/GAPDH Q-RT-PCR values of triplicate assays (mean +/- SD). B, Re-addition of AZD6244 blocks PLX4032 induction of ERK1/2 phosphorylation. 8505C cells were treated with PLX4032 for the indicated times and then re-treated with either fresh PLX4032 or MEK inhibitor AZD6244 for an additional 1, 6 or 24 h. Proteins were extracted and immunobloted for pERK and tERK
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362626726