Work

Supplementary Figure 1 from PD-1 Blockade Expands Intratumoral Memory T Cells

Year: 2023

Type: supplementary-materials

Abstract: Gating strategy used to analyze immune cell subpopulations within tumor infiltrating lymphocytes. Flow cytometry dot-plots on the left side and a dendrogram displaying the gating strategy of six populations on the right. We gated alive (7AAD negative) singlet cells that were positive for leukocyte antigen (CD45+). Then, the following gating was applied to designate NK cells (CD45+CD56+CD3-), B cells (CD45+CD19/CD20+CD3-), monocytes (CD45+CD14+), and T cells (CD45+CD3+). From the monocytes, a subset of MDSC with low HLA-DR positive cells was used. Finally, T regulatory cells (Treg) were described among CD4+ cells and from those, the CD25+HighCD127Low.

DOI: 10.1158/2326-6066.22537960.v1

Authors: Antoni Ribas, Daniel Sanghoon Shin, Jesse Zaretsky, Juliet Frederiksen, Andrew Cornish, Earl Avramis, Elizabeth Seja, Christine Kivork, Janet Siebert, Paula Kaplan-Lefko, Xiaoyan Wang, Bartosz Chmielowski, John A. Glaspy, Paul C. Tumeh, Thinle Chodon, Dana Pe'er, Begoña Comin-Anduix

Language: English

Topic: Cancer Immunotherapy and Biomarkers

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362628913

Cites:

Cited by:

Related to: 10