Supplementary Figures 1 -18 from Aberrant Glycosylation Promotes Lung Cancer Metastasis through Adhesion to Galectins in the Metastatic Niche
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Fig. 1. Galectin-3 is present within tumors. Supplementary Fig. 2. Analysis of peripheral blood from tumor-bearing mice by flow cytometry. Supplementary Fig. 3. Galectin-3 expression on CD11b+ cells is not dependent on the presence of tumors. Supplementary Fig. 4. Galectin-3 presentation on CD11b+ leukocytes is not carbohydrate dependent. Supplementary Fig. 5. Tumor conditioned medium induces galectin-3-independent mobilization of CD11b+galectin-3+ leukocytes. Supplementary Fig. 6. Galectin-3 is not necessary for mobilization of galectin-3+CD11b+ leukocytes or metastatic seeding. Supplementary Fig. 7. IL-6 induces mobilization of galectin-3+CD11b+ leukocytes. Supplementary Fig. 8. IL-6 induces the mobilization of galectin-3+CD11b+ granulocytes. Supplementary Fig. 9. Galectin-3 expression by leukocytes or stromal cells is not necessary for IL-6-dependent mobilization of myeloid cells. Supplementary Fig. 10. The Thomsen-Friedenreich Antigen is elevated in metastatic cells and human lung cancer tissue. Supplementary Fig. 11. Gene expression microarray analysis of glycosyltransferases shows no evidence of differential regulation of transferases that generate galectin-3 ligands. Supplementary Fig. 12. Differential regulation of select glycosyltransferase activity prevents glycan elongation and preserves T-Antigen presentation. Supplementary Fig. 13. O-linked core 1 disaccharide synthesis is unaltered during metastatic progression, but branching and elongation affect T-Antigen surface presentation. Supplementary Fig. 14. Transfection of Gcnt3 and knockdown of St6galnac4. (A) qRT-PCR for Gcnt3 in 393M1 cells following transfection with Gcnt3. Supplementary Fig. 15. Transfection of Gcnt3 reduces T-Antigen expression and adhesion to galectin-3. Supplementary Fig. 16. Knockdown of St6GalNAcIV reduces the formation of metastases in vivo. Supplementary Fig. 17. Knockdown of St6galnac4 does not affect proliferation. Supplementary Fig. 18. Galectin-3 is elevated in mice bearing tumors.
Language: English
Topic: Galectins and Cancer Biology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362636135