Supplementary Data Figure S1-S8 from A Genome-scale CRISPR Screen Identifies the ERBB and mTOR Signaling Networks as Key Determinants of Response to PI3K Inhibition in Pancreatic Cancer
Year: 2023
Type: supplementary-materials
Abstract: Figure S1. KRAS-dependency and effect of Cas9 expression on response to PI3K inhibition. Figure S2. Whole-genome CRISPR screen shows good replicate correlation and identifies enhancers and suppressors of GDC0941 antiproliferative activity. Figure S3. Expression of Cas9 does not alter response to BYL719 or GDC0941. Figure S4. Population doublings, replicate correlation and alternative analysis of the minipool screen. Figure S5. PI3K signalling is reduced following serum starvation. Figure S6. Combined inhibition of PI3K and mTOR results in potent antiproliferative activity in pancreatic cancer cell lines. Figure S7. Combined p110� and mTOR or ERBB-family inhibition leads to sustained suppression of S6 phosphorylation. Figure S8. CRISPR and drug combination screens implicate the ERBB-PI3K-MTOR signalling axis as a key vulnerability of pancreatic cancer cells.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362537543