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Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS

Year: 2023
Type: article
Abstract: Although RNA modifications play roles in many cellular and developmental processes in various domains of life, knowledge about the patterns and functions of RNA modifications in T. gondii is limited. Here, a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was used to study global RNA modifications in T. gondii strains of distinct virulence backgrounds. We quantified 22 modified nucleosides in both tRNA and sncRNA. Significant T. gondii strain-specific differences in RNA modifications were detected. More tRNA modifications correlated with T. gondii virulence proteins than sncRNA modifications. RNA modifications were significantly correlated with virulence proteins. Our data provide the first comprehensive profiling of the modifications tRNA and sncRNA in T. gondii, expanding the diversity of RNA modifications in this parasite and suggesting new regulators for modulating its virulence.
DOI: 10.1128/spectrum.03564-22
Authors: Wei Wang, Yi Yang, Huanping Guo, Ming-Han Li, Xiao-Qing Chen, Xin-Yu Wei, Yu Chen, Hany M. Elsheikha, Xiao-Xuan Zhang
Institutions: Qingdao Agricultural University, Heilongjiang Bayi Agricultural University, Zhejiang University, Army Medical University, Jilin Agricultural University, Jiangxi Agricultural University, University of Nottingham
Language: English
Subfield: Parasitology
Open Access status: gold
Estimated APC paid: $2,500
OpenAlex ID: W4363646590
FWCI: 0.935
Cites: 55
Cited by: 4
Related to: 10