Year: 2023
Type: article
Abstract: The regular incremental secretion of enamel and dentine can be interrupted during periods of stress resulting in accentuated growth lines. These accentuated lines, visible under light microscopy, provide a chronology of an individual's stress exposure. Previously, we showed that small biochemical changes along accentuated growth lines detected by Raman spectroscopy, coincided with the timing of medical history events and disruptions of weight trajectory in teeth from captive macaques. Here, we translate those techniques to study biochemical changes related to illness and prolonged medical treatment during early infancy in humans. Chemometric analysis revealed biochemical changes related to known stress-induced changes in circulating phenylalanine as well as other biomolecules. Changes in phenylalanine are also known to affect biomineralization which is reflected in changes in the wavenumbers of hydroxyapatite phosphate bands associated with stress in the crystal lattice. Raman spectroscopy mapping of teeth is an objective, minimally-destructive technique that can aid in the reconstruction of an individual's stress response history and provide important information on the mixture of circulating biochemicals associated with medical conditions, as applied in epidemiological and clinical samples.
Source: Chemosphere
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,610
Funders: National Science Foundation, Automotive Research Center, University of Sydney, National Institutes of Health, Radcliffe Institute for Advanced Study, Harvard University, Australian Research Council, National Health and Medical Research Council, National Institute of Environmental Health Sciences, National Center for Research Resources, National Center for Advancing Translational Sciences, California National Primate Research Center, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Office of Research Infrastructure Programs, National Institutes of Health
Awards: G1361149278, Early Life Environmental Toxicant Exposure and Oral Health, G2914888018, G3030616296, G4095048254, Longitudinal Investigation of Maternal Influences on Infant Outcomes Mediated by Physiological Investment and Behavioral Care during Lactation in Rhesus Macaques (Macaca mulatta), G5146737010, Vibrational Spectroscopy Microprobe/FESEM/AFM Imaging of Cells, Tissues and Materials, Biomarker of intrauterine metal exposure, G7149975552, Pushing the Boundaries of Multi-modal Biospectroscopic Microscopies, G791781759, Reconstructing fetal toxicant exposure and homeostatic disruptions, G9000274184, California National Primate Research Center
OpenAlex ID: W4364355484