Supplementary Figure S5 from Biomarker Data from the Phase III KATHERINE Study of Adjuvant T-DM1 versus Trastuzumab for Residual Invasive Disease after Neoadjuvant Therapy for HER2-Positive Breast Cancer
Year: 2023
Type: supplementary-materials
Abstract: Comparison of pre-NAT and post-NAT surgical RNA-evaluable samples. (A) ERBB2 (i.e., HER2), CD274 (i.e., PD-L1), checkpoint inhibitor signature (i.e., PD-L1/PD-L2/IDO), CD8A (i.e., CD8), TEFF signature CD8/granzymeA/granzymeB/perforin/IFNγ), three-gene signature (i.e., PD-L1/IFNγ/CXCL9), five-gene signature (i.e., PD-L1/granzymeB/CD8/IFNγ/CXCL9), Th1 cytokine signature (i.e., CXCL9/CXCL10/CXCL11), and B cell gene expression. (B) Frequency of AIMS PAM50 subtypes overall and by hormone receptor status. (C) Frequency of AIMS PAM50 subtypes by level of HER2 protein expression assessed by IHC in pre- and post-NAT samples. (D) Frequency of AIMS PAM50 subtypes by level of HER2 gene expression in pre- and post-NAT samples. (E) Hallmark gene sets enriched for genes upregulated in post-NAT surgical versus pre-NAT samples (adjusted P < 0.05). (F) Hallmark gene sets enriched for genes downregulated in post-NAT surgical versus pre-NAT samples (adjusted P < 0.05). Differential gene expression and pathway analyses were adjusted for tumor content. (G) Multidimensional scaling plot showing the differences in gene expression profiles between pre-NAT and post-NAT surgical samples. AIMS, Absolute Intrinsic Molecular Subtyping; ER, estrogen receptor; HER2, human epidermal growth factor receptor 2; HR, hormone receptor; IFNγ, interferon gamma; IHC, immunohistochemistry; MDS, multidimensional scaling; Med, median; NAT, neoadjuvant therapy; NEG, negative; NES, normalized enrichment score; PAM50, Prediction Analysis of Microarray 50; POS, positive; PR, progesterone receptor.
Authors: Carsten Denkert, Chiara Lambertini, Peter A. Fasching, Katherine L. Pogue-Geile, Max S. Mano, Michael Untch, Norman Wolmark, Chiun-Sheng Huang, Sibylle Loibl, Eleftherios P. Mamounas, Charles E. Geyer, Peter C. Lucas, Thomas Boulet, Chunyan Song, Gail D. Lewis, Malgorzata Nowicka, Sanne de Haas, Mark Basik
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4365510133