Supplementary Tables S1-S9 and Figures S1-S9 from Dimeric p53 Mutant Elicits Unique Tumor-Suppressive Activities through an Altered Metabolic Program
Year: 2023
Type: supplementary-materials
Abstract: Table S1 shows predicted off target sites for p53TD mutant mice. Table S2 shows upregulated PPAR target genes in p53AD vs p53-null. Table S3 shows ATAC-seq and RNA-seq congruent genes in p53AD vs p53 null. Table S4 shows p53half site analysis in PPAR target genes Table S5 shows control sequences used for p53 half site analysis Table S6 shows altered genes in p53AD-derived thymic lymphomas Table S7 shows primers used for genotyping of p53 mutant mice Table S8 shows primers used for qRT-PCR Table S9 shows antibodies used for flow cytometry Figure S1 shows extended data for generation of knock-in mouse models and features of p53TD mutant proteins and mice. Figure S2 shows homozygous mutant mice survival curves as well as tumor data. Figure S3 shows exome sequencing results for thymic lymphomas and hemangiosarcomas derived from p53 monomer and dimer mutant mice. Figure S4 shows characterization of stress responses of p53TD mutants. Figure S5 shows RNA-sequencing results for p53AD and p53RP, and Mdm2 genetic deletion rescue experiments. Figure S6 shows RNA-seq results of basal p53AD vs p53-null thymi, upstream regulator analysis, and ATAC-seq results for specific loci. Figure S7 shows extended data for p53 dimer mutant novel activities (co-IP, motif analysis, other dimer mutants activities, and tumor data) Figure S8 shows ferroptosis and scRNA-seq extended data Figure S9 shows diagram summarizing findings within this study.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4366140962