mRNA Localization and Local Translation of the Microtubule Severing Enzyme, Fidgetin-Like 2, in Polarization, Migration and Outgrowth
Year: 2023
Type: preprint
Abstract: SUMMARY Cell motility requires strict spatiotemporal control of protein expression. During cell migration, mRNA localization and local translation in subcellular areas like the leading edge and protrusions are particularly advantageous for regulating the reorganization of the cytoskeleton. Fidgetin-Like 2 (FL2), a microtubule severing enzyme (MSE) that restricts migration and outgrowth, localizes to the leading edge of protrusions where it severs dynamic microtubules. FL2 is primarily expressed during development but in adulthood, is spatially upregulated at the leading edge minutes after injury. Here, we show mRNA localization and local translation in protrusions of polarized cells are responsible for FL2 leading edge expression after injury. The data suggests that the RNA binding protein IMP1 is involved in the translational regulation and stabilization of FL2 mRNA, in competition with the miRNA let-7. These data exemplify the role of local translation in microtubule network reorganization during migration and elucidate an unexplored MSE protein localization mechanism. Graphical Abstract Highlights The microtubule severing enzyme FL2 RNA is localized to the leading edge FL2 mRNA localization leads to FL2 translation within protrusions The IMP family works in concert with Let-7 miRNA to regulate FL2 mRNA
Authors: Rayna Birnbaum, Jeetayu Biswas, Robert H. Singer, David J. Sharp
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: The sequence recognition, structure and function of the IMP family of mRNA binding proteins, A Novel Therapeutic that Harnesses Microtubules to Promote Cavernous Nerve Regeneration after Radical Prostatectomy, The microtubule cytoskeleton as a novel target for cavernous nerve regeneration after prostatectomy, Medical Scientist Training Program, Cancer Center Support Grant, Mechanism of mRNA Localization and Localized Translation in Neurons
OpenAlex ID: W4366283415