Work

A Modified Acyl-RAC Method of Isolating Retinal Palmitoyl Proteome for Subsequent Detection through LC-MS/MS

Year: 2023

Type: article

Abstract: C-radiolabeled palmitic acid and have many limitations, including poor sensitivity. Relatively recent studies use thiopropyl Sepharose 6B resin, which offers efficient detection of palmitoylated proteome but is now discontinued from the market. Here, we describe a modified acyl resin-assisted capture (Acyl-RAC) method using agarose S3 high-capacity resin to purify palmitoylated proteins from the retina and other tissues, which is greatly compatible with downstream processing by LC-MS/MS. Unlike other palmitoylation assays, the present protocol is easy to perform and cost-effective. Graphical abstract.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.4654

Authors: Sree Motipally, Boyden Myers, Emily Sechrest, David Sokolov, Joseph Murphy, Saravanan Kolandaivelu

Institution: West Virginia University

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: West Virginia University, National Institutes of Health, National Eye Institute, National Institute of General Medical Sciences