Work

Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures

Year: 2023

Type: article

Abstract: Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived from mice. We also describe additional steps for co-culture with mouse fibroblasts to enable the study of interactions with other cellular components of the tissue microenvironment. For complete details on the use and execution of this protocol, please refer to Martinez-Ordoñez et al. (2023).1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102243

Authors: Anxo Martinez-Ordoñez, Tania Cid-Diaz, Angeles Duran, Qixiu Han, Jorge Moscat, Maria T. Diaz-Meco

Institution: Weill Cornell Medicine

Language: English

Topic: Cancer Cells and Metastasis

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500