Characterization of tryptophan oxidation affecting D1 degradation by FtsH in the photosystem II quality control of chloroplasts
Year: 2023
Type: article
Abstract: Photosynthesis is one of the most important reactions for sustaining our environment. Photosystem II (PSII) is the initial site of photosynthetic electron transfer by water oxidation. Light in excess, however, causes the simultaneous production of reactive oxygen species (ROS), leading to photo-oxidative damage in PSII. To maintain photosynthetic activity, the PSII reaction center protein D1, which is the primary target of unavoidable photo-oxidative damage, is efficiently degraded by FtsH protease. In PSII subunits, photo-oxidative modifications of several amino acids such as Trp have been indeed documented, whereas the linkage between such modifications and D1 degradation remains elusive. Here, we show that an oxidative post-translational modification of Trp residue at the N-terminal tail of D1 is correlated with D1 degradation by FtsH during high-light stress. We revealed that Arabidopsis mutant lacking FtsH2 had increased levels of oxidative Trp residues in D1, among which an N-terminal Trp-14 was distinctively localized in the stromal side. Further characterization of Trp-14 using chloroplast transformation in Chlamydomonas indicated that substitution of D1 Trp-14 to Phe, mimicking Trp oxidation enhanced FtsH-mediated D1 degradation under high light, although the substitution did not affect protein stability and PSII activity. Molecular dynamics simulation of PSII implies that both Trp-14 oxidation and Phe substitution cause fluctuation of D1 N-terminal tail. Furthermore, Trp-14 to Phe modification appeared to have an additive effect in the interaction between FtsH and PSII core in vivo. Together, our results suggest that the Trp oxidation at its N-terminus of D1 may be one of the key oxidations in the PSII repair, leading to processive degradation by FtsH.
Source: eLife
Institutions: Setsunan University, Okayama University, Institute of Plant Science and Resources, Okayama University, Research Institute for Interdisciplinary Science, Okayama University, Research Center for Advanced Science and Technology, The University of Tokyo, The University of Tokyo, Chinese Academy of Sciences, Institute of Himalayan Bioresource Technology, Center for Excellence in Molecular Plant Sciences, Council of Scientific and Industrial Research, University of Münster, Centre National de la Recherche Scientifique, Sorbonne Université, Institut de Biologie Physico-Chimique, Université Pierre-et-Marie-Curie
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Awards: Thylakoid Membrane Remodeling and Optimization of Photosynthetic Activity, G1604788261, G1707236070, G2140024934, G230760944, G2847163159, G2878895784, G2985377510, prediction of proton transfer in proteins based on hydrogen-bond network structures, G3794970729, G3802524579, G4244104215, G4696816355, G4906035352, Protein quality control of photosystemI in thylakoid membranes, G5151641245, Asymmetric electron transfer in photosynthetic reaction centers, G5192185249, G6148573568, G641627975, G676061642, G6919872028, G7057597699, G7497639852, Dynamics of the structure and function of photosystem I complex in response to environmental changes, G8788570523, Dynamique des membranes transductrices d'énergie : biogénèse et organisation supramoléculaire.
OpenAlex ID: W4385289643