Work

PC37. The Effect of Local Exosomes, Stem Cells and Tacrolimus on Neurite Extension

Year: 2023

Type: article

Abstract: PURPOSE: The aim of this study was to investigate the combined effect of local delivery of Purified Exosome Product (PEP), mesenchymal stem cells (MSCs) and Tacrolimus (FK506) alone and combined on nerve regeneration. METHODS: A 3D in vitro system was used to evaluate regenerating neurites from a rat neonatal dorsal root ganglion into the adjacent nerve. Decellularized allografts were augmented with local application of (i) MSCs, (ii) PEP (5%), (iii) FK506 (100 ng/mL), (iv) combined PEP+FK506, and (v) MSC+FK506 (N=9/group). Outcomes were compared to untreated autografts and allografts. After 48 hours, constructs were stained against Neurofilament-160 to measure neurite extension, and CD90-DAPI to confirm stem cell characterization. RESULTS: Stem cell viability was confirmed in all MSC-treated grafts using CD90. Combined PEP+FK506 treatment to allografts resulted in the longest neurite extension and significantly improved outcomes of untreated allografts (p<0.05), reaching comparability to autografts (p=0.66). In allografts, combined PEP+FK506 treatment was comparable to PEP alone and MSC+FK506 (respectively, p=0.97 and p=0.80). PEP alone and MSC+FK506 treatment were found superior to negative controls (P<0.05). CONCLUSION: PEP+FK506 treatment in allografts resulted in the longest neurite extension, was superior to untreated allografts, and comparable to autograft, PEP alone and MSC+FK506 treatment. These results suggest that the neuroregenerative effect of PEP is similar to MSCs. PEP could overcome the limitations of harvesting, culturing and seeding of stem cells, making this more translatable to clinical care. In vivo animal models are needed to further investigate the effect of PEP alone and combined with FK506 in nerve regeneration.

Source: Plastic & Reconstructive Surgery Global Open

DOI: 10.1097/01.gox.0000934508.60378.fd

Authors: Tara Sara Saffari, Daan J. Rademakers, Tiam M. Saffari, Alexander Y. Shin

Institution: Mayo Clinic

Language: English

Topic: Nerve injury and regeneration

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,195