Work

Ligand-directed labeling of opioid receptors for covalent attachment of fluorophores or small-molecule probes

Year: 2023

Type: article

Abstract: This protocol describes endogenous labeling of opioid receptors (ORs) using a ligand-directed reagent, naltrexamine-acylimidazole compounds (NAI-X). NAI acts by guiding and permanently tagging a small-molecule reporter (X)—such as fluorophores or biotin—to ORs. Here we detail syntheses and uses of NAI-X for OR visualization and functional studies. The NAI-X compounds overcome long-standing challenges in mapping and tracking endogenous ORs as the labeling can be done in situ with live tissues or cultured cells. For complete details on the use and execution of this protocol, please refer to Arttamangkul et al.1,2

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102231

Authors: Hayden Adoff, Victoria S. Halls, Emily Holland, Braden Lobingier, Seksiri Arttamangkul

Institutions: Oregon Health & Science University, Vollum Institute

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500