Work

Estudio de estabilidad acelerada del ingrediente farmacéutico activo Bm86/pP0 por el método de arrhenius

Year: 2023

Type: article

Abstract: The study of protein stability is currently an essential process for the development of vaccine products that contain these as an active ingredient. This work was carried out at the Center for Genetic Engineering and Biotechnology of Camagüey, where the Technological Development area is currently working on the design of a vaccine against ticks. The objective of this work was to determine the inactivation time of the protein Bm86 carrying the peptide P0, applying accelerated studies of heat stress in order to predict the stability of the vaccine against ticks, based on the Arrhenius equation. Different studies were also carried out to analyze the possible inactivation pathways of the Bm86 protein. The results obtained indicated that at a storage temperature between 2 °C and 8 °C, the protein, as an active pharmaceutical ingredient, has a predictive duration time of approximately 2.5 years before 10% of it is inactivated.

Source: Nexo Revista Científica

DOI: 10.5377/nexo.v36i02.15993

Authors: Omar Díaz González, Roxana Barrios Taura, Rutdali María Segura Silva, Laritza Caridad Domínguez Rabilero, Amaury Pérez Sánchez

Institutions: Centro de Ingeniería Genética y Biotecnología, University of Camagüey

Language: Spanish

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4367057297

FWCI:

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