Year: 2023
Type: article
Abstract: Recursive splicing is a non-canonical splicing mechanism in which an intron is removed in segments via multiple splicing reactions. Relatively few recursive splice sites have been identified with high confidence in human introns, and more comprehensive analyses are needed to better characterize where recursive splicing happens and whether or not it has a regulatory function. In this study, we use an unbiased approach using intron lariats to search for recursive splice sites in constitutive introns and alternative exons in the human transcriptome. We find evidence for recursive splicing in a broader range of intron sizes than previously reported and detail a new location for recursive splicing at the distal ends of cassette exons. In addition, we identify evidence for the conservation of these recursive splice sites among higher vertebrates and the use of these sites to influence alternative exon exclusion. Together, our data demonstrate the prevalence of recursive splicing and its potential influence on gene expression through alternatively spliced isoforms.
Source: Life Science Alliance
Authors: Emma R. Hoppe, Dylan B. Udy, Robert K. Bradley
Institutions: University of Washington, Fred Hutch Cancer Center
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,500
Funders: U.S. Department of Defense, Leukemia and Lymphoma Society, Fred Hutchinson Cancer Research Center, Mark Foundation For Cancer Research, Paul G. Allen Frontiers Group, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases
Awards: G1305388156, G1929525408, G3992978787, G4119534169, G4266498966, U2AF1 mutations in myelodysplastic syndromes: from mechanism to therapy, G478030013, G5311849004, Training in Cellular and Molecular Biology, G5850682332, Interrogating the minor spliceosome to understand and treat leukemia, G7448966117, G887357334, G8956203657, G9000085113, Cancer Center Support Grant, Genetic and molecular basis for SRSF2 mutations in myelodysplasia, Functional and molecular consequences of SF3B1 mutations in human hematopoietic stem cells, G976159415
OpenAlex ID: W4367857221