Work

Implementation of a Drug Screening Platform to Target Gch1 Expression in Injured Mouse Dorsal Root Ganglion Neurons

Year: 2023

Type: article

Abstract: expression in individual injured dorsal root ganglion (DRG) neurons in vitro, we can screen for compounds that regulate its expression levels. This approach also allows us to gain valuable biological insights into the pathways and signals regulating GCH1 and BH4 levels upon nerve injury. This protocol is compatible with any transgenic reporter system in which the expression of an algesic gene (or multiple genes) can be monitored fluorescently. Such an approach can be scaled up for high-throughput compound screening and is amenable to transgenic mice as well as human stem cell-derived sensory neurons. Graphical overview.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.4666

Authors: Shane Cronin, Lance Davidow, Anthony Arvanites, Lee Rubin, Josef Penninger, Clifford Woolf

Institutions: Boston Children's Hospital, Harvard University, Harvard Stem Cell Institute, Institute of Molecular Biotechnology

Language: English

Topic: Pain Mechanisms and Treatments

Subfield: Physiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Institute of Neurological Disorders and Stroke, Eunice Kennedy Shriver National Institute of Child Health and Human Development