Year: 2023
Type: article
Abstract: ABSTRACT The programmable nature of DNA allows the construction of custom-designed static and dynamic nanostructures, and assembly conditions typically require high concentrations of magnesium ions which restricts their applications. In other solution conditions tested for DNA nanostructure assembly, only a limited set of divalent and monovalent ions have been used so far (typically Mg 2+ and Na + ). Here, we investigate the assembly of DNA nanostructures in a wide variety of ions using nanostructures of different sizes: a double-crossover motif (76 bp), a three-point-star motif (∼134 bp), a DNA tetrahedron (534 bp) and a DNA origami triangle (7221 bp). We show successful assembly of a majority of these structures in Ca 2+ , Ba 2+ , Na + , K + and Li + and provide quantified assembly yields using gel electrophoresis and visual confirmation of a DNA origami triangle using atomic force microscopy. We further show that structures assembled in monovalent ions (Na + , K + and Li + ) exhibit up to a 10-fold higher nuclease resistance compared to those assembled in divalent ions (Mg 2+ , Ca 2+ and Ba 2+ ). Our work presents new assembly conditions for a wide range of DNA nanostructures with enhanced biostability.
Source: Small
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funders: National Science Foundation, National Institutes of Health, University at Albany, National Institute on Alcohol Abuse and Alcoholism, National Institute on Aging, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering, National Institute of Dental and Craniofacial Research, Division of Chemistry
Awards: Detection and Automatic Privacy-Protected Contact Tracing System Designed for COVID-19, G2791503005, DNA Nanostructures as siRNA Delivery Vehicles for Alzheimer's Therapy, G3707745664, CAREER: Chemical Biology Approaches to Study 5-Methylcytidine Derivatives in RNA and Develop CRISPR-Tet2-Based Epitranscriptomic Tool for Gene Regulation, G4342460786, RAPID: A rapid and ultrasensitive technology for sensing intact SARS-CoV-2 using designer DNA nanostructure capture probes and photonic resonator interference scattering microscopy, A Rapid and Sensitive Technology for Direct Sensing of Intact SARS-CoV-2 Virions Using Designer DNA Nanostructure Probes and a Smartphone Fluorimeter, G5907858109, Designer DNA Nanostructure Based Biosensing for Rapid COVID19 Detection and Monitoring using Saliva Sample, G863430095, Ultrasensitive HIV viral load quantitation using designer DNA nanostructure capture probes and photonic resonator interference scattering microscopy, Chemical Modifications to Wobble Uridines in tRNA Regulate Responses to Stress
OpenAlex ID: W4379093628