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User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology (Adv. Mater. 19/2023)

Year: 2023

Type: article

Abstract: Multiplexed Cell Capture Harvesting cells from biomaterials for downstream analysis without perturbing their state represents an outstanding challenge in 3D/4D culture and tissue engineering. In article number 2209904, Cole A. DeForest and co-workers demonstrate that engineered sortase transpeptidases can be used to selectively degrade and recover single-cell suspensions from specific subvolumes of hydrogel multimaterials, permitting multiplexed cell capture with largely “biologically invisible” cues.

Source: Advanced Materials

DOI: 10.1002/adma.202370132

Authors: Ross C. Bretherton, Amanda J. Haack, Irina Kopyeva, Fariha Rahman, Jonah D. Kern, Darrian Bugg, Ashleigh B. Theberge, Jennifer Davis, Cole A. DeForest

Institution: University of Washington

Language: English

Topic: Hydrogels: synthesis, properties, applications

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W4376143248

FWCI:

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