Work

More than magnetic isolation: Dynabeads as strong Raman reporters towards simultaneous capture and identification of targets

Year: 2023

Type: preprint

Abstract: m area imaging using 0.5 s, 7 mW laser acquisition with single and clustered beads providing a 44- and 68-fold larger Raman intensity compared to signature from cells. Higher polystyrene and iron oxide content in clusters yields larger signal intensity and conjugation to bacteria strengthens clustering as a bacterium can bind to more than one bead as observed via transmission electron microscopy (TEM). Our findings shed light on the intrinsic Raman reporter nature of Dynabeads. When combined with emerging techniques for the separation of target-bound Dynabeads from unbound Dynabeads such as using centrifugation through a density media bi-layer, they have potential to demonstrate their dual function for target isolation and detection without tedious staining steps or unique plasmonic substrate engineering, advancing their applications in heterogeneous samples like food, water, and blood.

Source: PubMed

DOI: 10.48550/arxiv.2305.07199

Authors: Lee, Jongwan, McDonald, Marissa, Mhlanga, Nikiwe, Kang, Jeon Woong, Karnik, Rohit, Tadesse, Loza F.

Institution: Massachusetts Institute of Technology

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, National Institutes of Health, Abdul Latif Jameel Water and Food Systems Lab, Massachusetts Institute of Technology, National Cancer Institute, Division of Materials Research

Award: G2056243947

OpenAlex ID: W4376632487

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