Work

Anomalous DNA hybridisation kinetics on gold nanorods revealed via a dual single-molecule imaging and optoplasmonic sensing platform

Year: 2023

Type: article

Abstract: towards high binding site occupancies). A decrease in the association rate over the measurement duration is reported. Our dual optoplasmonic sensing and imaging platform offers insight into the observed phenomenon, revealing that irreversible hybridisation events accumulate over detected step signals in optoplasmonic sensing. Our results point to novel physicochemical mechanisms that result in the stabilisation of DNA hybridisation on optically-excited plasmonic nanoparticles.

Source: Nanoscale Horizons

DOI: 10.1039/d3nh00080j

Authors: Narima Eerqing, Hsin-Yu Wu, Sivaraman Subramanian, Serge Vincent, Frank Vollmer

Institution: University of Exeter

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funder: Engineering and Physical Sciences Research Council

Awards: G1664034006, EPSRC Centre for Doctoral Training in Electromagnetic Metamaterials, Molecular Mechanics of Enzymes, An Optical Single Molecule Scanner of Protein Motion