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Fluorosulfate as a Latent Sulfate in Peptides and Proteins

Year: 2023
Type: article
Abstract: Sulfation widely exists in the eukaryotic proteome. However, understanding the biological functions of sulfation in peptides and proteins has been hampered by the lack of methods to control its spatial or temporal distribution in the proteome. Herein, we report that fluorosulfate can serve as a latent precursor of sulfate in peptides and proteins, which can be efficiently converted to sulfate by hydroxamic acid reagents under physiologically relevant conditions. Photocaging the hydroxamic acid reagents further allowed for the light-controlled activation of functional sulfopeptides. This work provides a valuable tool for probing the functional roles of sulfation in peptides and proteins.
DOI: 10.1021/jacs.3c07937
Authors: Chao Liu, Xueyi Liu, Mi Zhou, Chaoshuang Xia, Yuhan Lyu, Qianni Peng, Chintan Soni, Zefeng Zhou, Qiwen Su, Yujia Wu, Eranthie Weerapana, Jianmin Gao, Abhishek Chatterjee, Cheng Lin, Jia Niu
Language: English
Open Access status: green
Funders: National Science Foundation, Boston College, National Institutes of Health, National Human Genome Research Institute, NIH Office of the Director, National Institute of General Medical Sciences, National Center for Chronic Disease Prevention and Health Promotion, Division of Chemistry
Awards: CryoProbe-enabled Bruker AVANCE NEO 500 MHz NMR Spectrometer, G1726345278, G282645812, G5217627585, Development of Compact CRISPR Editors for Multiplexed Editing of Complex Gene Networks, G6884064952, MRI: Acquisition of a CryoProbe-Enabled NMR Spectrometer for Enhanced Efficiency and Sensitivity in Chemical and Biological Research, G8869753781, A genetically encoded toolset to decipher the biology of post-translational modifications in the mammalian proteome
OpenAlex ID: W4386272374
FWCI: 1.153
Cites: 59
Cited by: 13
Related to: 10