Figure S3 from Type I Interferon Signaling via the EGR2 Transcriptional Regulator Potentiates CAR T Cell–Intrinsic Dysfunction
Year: 2023
Type: other
Abstract: Impact of EGR2 knockout on different T-cell subsets and CD28-based CAR T-cells. A, Naive, central memory, effector memory, and effector T-cells were magnetically isolated. PSMA CAR-T cells with AAVS1 and EGR2 knockout (KO) were then generated from the T-cell subsets and restimulation experiments were conducted to examine the development of exhaustion and memory. B, Expression of exhaustion markers (TIM3+LAG3+), C, cytotoxicity and D, expression of early memory markers (CD45RO+CD27+) after chronic CAR stimulation. (E-H) EGR2 was deleted in PSMA-CD28 based CAR T-cells, and the immunophenotype of CAR T-cells was assessed during the restimulation assay. E, Expression of exhaustion markers (TIM3+LAG3+), F, cytotoxicity, G, expression of early memory markers (CD45RO+CD27+) after chronic CAR stimulation and H, CAR T-cell expansion capacity during restimulation. All experiments were conducted using T-cells from two independent healthy donors, and panels (A-H) are representative data from one donor. (Mann-Whitney test, n = 4). *P < 0.05, **P < 0.01, ***P < 0.001, ns.: not significant.
Authors: In-Young Jung, Robert L. Bartoszek, Andrew J. Rech, Sierra M. Collins, Soon-Keat Ooi, Erik F. Williams, Caitlin R. Hopkins, Vivek Narayan, Naomi B. Haas, Noelle V. Frey, Elizabeth O. Hexner, Donald L. Siegel, Gabriela Plesa, David L. Porter, Adrian Cantu, John K. Everett, Sonia Guedan, Shelley L. Berger, Frederic D. Bushman, Friederike Herbst, Joseph A. Fraietta
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4379880732