Year: 2023
Type: article
Abstract: Recycling of membrane proteins enables the reuse of receptors, ion channels and transporters. A key component of the recycling machinery is the endosomal sorting complex for promoting exit 1 (ESCPE-1), which rescues transmembrane proteins from the endolysosomal pathway for transport to the trans-Golgi network and the plasma membrane. This rescue entails the formation of recycling tubules through ESCPE-1 recruitment, cargo capture, coat assembly and membrane sculpting by mechanisms that remain largely unknown. Herein, we show that ESCPE-1 has a single-layer coat organization and suggest how synergistic interactions between ESCPE-1 protomers, phosphoinositides and cargo molecules result in a global arrangement of amphipathic helices to drive tubule formation. Our results thus define a key process of tubule-based endosomal sorting.
Institutions: CIC bioGUNE, University of Basel, National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Ikerbasque, Universidad de La Rioja, Barcelona Supercomputing Center, Instituto de Ciencias de la Vid y del Vino, Consejo Superior de Investigaciones Científicas, University of the Basque Country, Instituto Biofisika
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $11,690
OpenAlex ID: W4380785248