Figure 3 from Proteomic Profiling of Extracellular Matrix Components from Patient Metastases Identifies Consistently Elevated Proteins for Developing Nanobodies That Target Primary Tumors and Metastases
Year: 2023
Type: other
Abstract: A-C, Sixty-seven proteins present in the metastasis-associated ECM signature shared among the 6 patient samples, ranked on the basis of their intensities (mean of the two duplicate ECM-enriched samples). Plots for colorectal cancer liver metastasis patient 1 (A), TNBC liver metastasis patient 1 (B), and TNBC lung metastasis patient 1 (C). Some ECM proteins are marked including FN (blue) and those tested for IHC in D. TNC, red; TGFBI, orange; POSTN, green. See also Supplementary Table S2 with rankings. D, IHC on a colorectal cancer liver metastasis sample shows that expression of TNC, TGFBI, and POSTN is restricted to the metastatic lesion (T) and absent from adjacent normal liver tissue (N). Arrows, regions of positive fiber-like staining. See also Supplementary Fig. S3. E, Testing expression of TNC in a multi-organ patient metastatic tissue array by IHC, with TNC-specific monoclonal antibody. Array contains two biopsy samples from each patient (N = 104). F, Scoring of TMA for stromal positivity of TNC, indicating patients that were negative (gray), patients with a single positive biopsy (light blue), and patients with both biopsies positive (dark blue). Inset shows percentage of positive biopsies that were metastases from lymph nodes at the target organs. G, Representative examples of metastases from E that were positive for TNC expression from four different primary tumor types (origin) to different metastatic sites. Arrows, positive fiber-like staining. LN, lymph node. CRC, colorectal cancer.
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4380790253