Figure 1 from Proteomic Profiling of Extracellular Matrix Components from Patient Metastases Identifies Consistently Elevated Proteins for Developing Nanobodies That Target Primary Tumors and Metastases
Year: 2023
Type: other
Abstract: Schematic of ECM enrichment and proteomic analysis of patient metastasis samples for matrisome nanobody library construction and panning. A, Patient metastasis samples used for ECM enrichment and proteomics for identification of ECM proteins/signatures associated with patient metastases. CRC, colorectal cancer. B, Immunoblots showing sequential extraction steps (1–5; see Materials and Methods) to obtain the final insoluble ECM-enriched pellet. C, Simplified schematic of nanobody library construction and isolation of nanobodies against ECM proteins identified from proteomics signatures. (See also Supplementary Fig. S1 for details on immunogen and immunization schedule.) D, Simplified schematic of LC-MS/MS–based proteomics pipeline to characterize the matrisome of the human metastases.
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4380790281