Work

Profiling milk oligosaccharides in Philippine buffalo breeds across lactation and identification of novel high-molecular weight fucosylated and sialylated oligosaccharides

Year: 2023

Type: article

Abstract: Animal milk oligosaccharides have attracted considerable interest because some sources can mimic the diversity of oligosaccharides found in human milk, associated with many health benefits. This study is the first to characterize buffalo milk oligosaccharides across lactation from representative Philippine breeds and crosses: traditionally draft-purpose Philippine Carabao, dairy-type Bulgarian Murrah, and their 50%, 75%, 87%, 93% crossbreeds. An optimized solid-phase extraction method combined with nano-LC-chip/QTOF mass spectrometry was developed, generating a bioinformatic library of 66 oligosaccharides. Fourteen fucosylated and 9 fucosylated-sialylated oligosaccharides were identified, with 22 novel oligosaccharides not previously found in human or bovine milk. The 93% crossbreed and Philippine Carabao breed had higher numbers of fucosylated oligosaccharides and novel oligosaccharides overall. The 93% crossbreed had higher diversity and total and sialylated oligosaccharides abundances across lactation stages. This is the most comprehensive study on buffalo milk oligosaccharides to date and indicates a strong potential for use in infant formulas.

Source: International Dairy Journal

DOI: 10.1016/j.idairyj.2023.105731

Authors: Dianne F. Manabat, Mrittika Bhattacharya, Gladys C. Completo, Daniela Barile

Institutions: University of the Philippines Los Baños, University of California, Davis

Language: English

Topic: Infant Nutrition and Health

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,430