The overlap of skin and blood T-cell clones in early-stage mycosis fungoides
Year: 2023
Type: dataset
Abstract: Mycosis fungoides (MF) is the most common subtype of cutaneous T-cell lymphoma that initially starts in the skin but can progress to involve blood with significant mortality in late stages. The presence of a dominant T-cell clone in the blood of early-stage MF appears to be associated with a poor prognosis. However, the identity of dominant blood T-cell clones and the degree of overlap between T-cell repertoires of blood and skin in MF have not been fully interrogated. Here, we evaluated the relationship of blood and skin T-cell repertoires and their relevance to prognosis in MF. We used high-throughput sequencing to interrogate the T-cell receptor (TCR) complementarity determining region 3 sequences in blood and skin of MF patients at the Jefferson Cutaneous Lymphoma Clinic (Adaptive Biotechnologies). ImmunoSEQ Analyzer provided T-cell repertoire overlap metric (Morisita’s index) and diversity measure (Simpson’s clonality score). Time to systemic treatment (TTST) was calculated as the time from initial diagnosis to initiation of first systemic therapy. 60 MF patients with no blood involvement were enrolled. 28% had a dominant clone in blood; of these, 18% were identical to dominant skin clones and 82% were distinct from the dominant clones identified in skin. We found that MF patients with discordant dominant TCR clones in blood and skin had a longer TTST when compared to the identical cohort (P=0.0057). Furthermore, patients with discordant dominant clones had a lower degree of T-cell repertoire overlap between blood and skin (P<0.0001) and higher blood T-cell repertoire diversity scores (P=0.013) when compared to the identical group. Our data shows that MF patients with dominant clones in blood segregate into two cohorts with distinct prognoses based on the identity of their peripheral T-cell clones.
Source: immuneACCESS
DOI: 10.21417/jd2023ba
Authors: D Joffe, L Banner, S Bhatti, R Zaya, L Gleason, A Mishra, L Kirsch, P Porcu, N Nikbakht
Language: English
Subfield: Dermatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4381327488